Journal: PLoS ONE
Article Title: IL-28B Genetic Variants Determine the Extent of Monocyte-Induced Activation of NK Cells in Hepatitis C
doi: 10.1371/journal.pone.0162068
Figure Lengend Snippet: NK cells from HCV(+) patients (C/C, n = 10; C/T, n = 9; T/T, n = 12) (A) and healthy donors (C/C, n = 4; C/T, n = 6; T/T, n = 4) (B) were co-cultured with autologous monocytes in the presence of R848 and then tested for IFN-γ production of CD56 Bright NK cells following co-incubation with HuH7 HCV Replicon cells. Purified HCV(+) monocytes were stimulated with R848 and then analysed with respect to expression (MFI) of TLR-7(non-T/T, n = 5; T/T, n = 4) (C) , TLR-8 (non-T/T, n = 5; T/T, n = 4) (D), and phosphorylated Stat-1 and Stat-5 (non-T/T, n = 5; T/T, n = 4) (E) , respectively.
Article Snippet: Then, cells were fixed and permeabilized using Cytofix/Cytoperm (BD Biosciences, Germany), followed by intracellular staining with anti-TLR-7 (ebioscience, USA) /anti-TLR-8 (BD, Germany) mAbs and flow cytometric analysis.
Techniques: Cell Culture, Incubation, Purification, Expressing